Journal:

Article Title: Identification of Mycobacterium Species by Multiple-Fluorescence PCR-Single-Strand Conformation Polymorphism Analysis of the 16S rRNA Gene

doi: 10.1128/JCM.39.9.3085-3091.2001

Figure Lengend Snippet: Average electrophoretic mobility values of the peaks detected by the fluorescence-labeled primers, in order of retention times, for all additional strain

Article Snippet: SSCP pattern determination based on electrophoretic mobility of PCR product a table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species group and species Strain No. of tests run Avg relative mobility 91E 264 ER10 ER11G RW01 13B 515 806R Slow growers M. asiaticum ATCC 25276T 2 87.1 103.3 221.2 257.5 182.0 228.1 230.3 314.6 M. branderi ATCC 51789T 2 87.7 101.6 232.2 264.7 181.4 234.1 242.3 323.5 M. celatum ATCC 51131T 2 88.8 106.7 237.3 268.8 182.1 232.8 242.1 323.3 M. flavescens ATCC 14474T 1 89.0 106.7 233.2 267.1 182.4 232.0 243.5 310.7 M. gastri ATCC 15754T 2 87.6 105.1 218.8 256.4 182.1 228.0 229.8 314.7 M. lentiflavum ATCC 51985T 1 87.4 103.9 230.2 265.3 182.3 231.7 236.0 295.9 M. malmoense ATCC 29571T 2 87.5 105.3 218.9 256.4 155.6 232.6 230.5 317.5 M. nonchromogenicum ATCC 19531 2 82.4 106.2 228.5 263.4 187.5 221.1 234.3 317.6 M. scrofulaceum ATCC 19981T 2 87.1 105.1 219.5 260.6 182.5 232.9 230.8 314.5 M. shimoidei ATCC 27962T 2 87.4 104.8 228.3 262.4 183.4 234.8 238.7 313.2 M. simiae ATCC 25275T 2 87.3 103.3 222.8 259.1 182.5 232.8 235.9 296.3 M. szulgai ATCC 35799T 2 87.2 105.1 221.5 257.3 181.8 228.3 230.3 314.6 M. triplex ATCC 700071T 1 87.7 103.5 223.1 262.3 182.4 234.4 235.9 296.8 M. triviale ATCC 23292T 2 82.4 106.4 220.9 263.7 182.8 232.1 237.5 313.2 M. ulcerans ATCC 19423T 1 88.1 105.5 218.7 261.2 182.1 220.3 230.9 314.9 Rapid growers M. abscessus ATCC 19977T 2 88.3 104.5 218.0 247.7 188.8 232.0 244.2 308.0 M. mucogenicum ATCC 49650T 1 87.0 103.5 226.2 259.2 183.0 231.6 244.7 308.7 M. peregrinum ATCC 14467T 2 86.1 102.3 226.8 257.1 182.4 232.4 243.3 307.5 M. smegmatis ATCC 19420T 2 87.0 103.3 226.0 260.8 182.4 232.2 243.6 307.3 M. thermoresistibile ATCC 19527T 2 86.9 103.1 228.9 266.4 186.4 221.9 243.0 308.8 Open in a separate window Average electrophoretic mobility values of the peaks detected by the fluorescence-labeled primers, in order of retention times, for all additional strain Preparation of DNA.

Techniques: Fluorescence

Journal:

Article Title: Antibiotic Susceptibility Profile of Bifidobacteria as Affected by Oxgall, Acid, and Hydrogen Peroxide Stress ▿

doi: 10.1128/AAC.00261-06

Figure Lengend Snippet: MICs of antibiotics for bifidobacteria

Article Snippet: Meanwhile, one strain developed slight resistance to ampicillin and two developed slight resistance to cloxacillin, but none developed resistance to penicillin G. table ft1 table-wrap mode="anchored" t5 TABLE 5. caption a7 Organism MIC (μg/ml) b of the following antibiotic c for the indicated organism: Amp Clo Pen Van Kan Neo Par Str Chl Ery Tet Nis B. animalis ATCC 27536 3.9 3.9 0.98 >500 500 500 500 250 3.9 0.98 62.5 0.98 B. bifidum R 0071 0.98 31.2 0.98 >500 500 125 125 250 1.9 500 62.5 0.98 B. bifidum ATCC 15696 Did not survive H 2 O 2 stressing B. bifidum BB12 0.98 1.9 0.98 >500 500 250 500 250 1.9 0.98 7.8 0.98 B. breve ATCC 15700 3.9 3.9 0.98 >500 >500 250 500 500 1.9 0.98 31.2 0.98 B. breve R0070 0.98 15.6 0.98 >500 500 250 250 250 1.9 0.98 7.8 0.98 B. infantis ATCC 15697 Did not survive H 2 O 2 stressing B. longum ATCC 15707 0.98 0.98 0.98 >500 500 250 500 62.5 1.9 0.98 7.8 0.98 B. longum ATCC 15708 3.9 7.8 0.98 >500 500 250 250 500 3.9 0.98 31.2 0.98 B. longum R0175 1.9 31.2 0.98 >500 500 125 250 250 1.9 0.98 15.6 1.9 B. longum P/N 601377 0.98 31.2 0.98 >500 250 125 125 125 1.9 1.9 125 0.98 B. pseudolongum ATCC 25562 Did not survive H 2 O 2 stressing B. thermophilum ATCC 25866 3.9 7.8 0.98 >500 500 500 500 500 1.9 0.98 15.6 0.98 Open in a separate window a Organisms were exposed for 90 min to H 2 O 2 at the MIC prior to 18 h of anaerobic growth in MRS broth, pH 6.5. b Median of four repetitions. c Amp, ampicillin; Clo, cloxacillin; Pen, penicillin G; Van, vancomycin; Kan, kanamycin; Neo, neomycin; Par, paromomycin; Str, streptomycin; Chl, chloramphenicol; Ery, erythromycin; Tet, tetracycline hydrochloride; Nis, nisin A. MICs of antibiotics for hydrogen peroxide-stressed a bifidobacteria The MICs of vancomycin and kanamycin did not change for any strain after H 2 O 2 treatment.

Techniques:

Journal:

Article Title: Antibiotic Susceptibility Profile of Bifidobacteria as Affected by Oxgall, Acid, and Hydrogen Peroxide Stress ▿

doi: 10.1128/AAC.00261-06

Figure Lengend Snippet: MICs of antibiotics for acid-stressed a bifidobacteria

Article Snippet: Meanwhile, one strain developed slight resistance to ampicillin and two developed slight resistance to cloxacillin, but none developed resistance to penicillin G. table ft1 table-wrap mode="anchored" t5 TABLE 5. caption a7 Organism MIC (μg/ml) b of the following antibiotic c for the indicated organism: Amp Clo Pen Van Kan Neo Par Str Chl Ery Tet Nis B. animalis ATCC 27536 3.9 3.9 0.98 >500 500 500 500 250 3.9 0.98 62.5 0.98 B. bifidum R 0071 0.98 31.2 0.98 >500 500 125 125 250 1.9 500 62.5 0.98 B. bifidum ATCC 15696 Did not survive H 2 O 2 stressing B. bifidum BB12 0.98 1.9 0.98 >500 500 250 500 250 1.9 0.98 7.8 0.98 B. breve ATCC 15700 3.9 3.9 0.98 >500 >500 250 500 500 1.9 0.98 31.2 0.98 B. breve R0070 0.98 15.6 0.98 >500 500 250 250 250 1.9 0.98 7.8 0.98 B. infantis ATCC 15697 Did not survive H 2 O 2 stressing B. longum ATCC 15707 0.98 0.98 0.98 >500 500 250 500 62.5 1.9 0.98 7.8 0.98 B. longum ATCC 15708 3.9 7.8 0.98 >500 500 250 250 500 3.9 0.98 31.2 0.98 B. longum R0175 1.9 31.2 0.98 >500 500 125 250 250 1.9 0.98 15.6 1.9 B. longum P/N 601377 0.98 31.2 0.98 >500 250 125 125 125 1.9 1.9 125 0.98 B. pseudolongum ATCC 25562 Did not survive H 2 O 2 stressing B. thermophilum ATCC 25866 3.9 7.8 0.98 >500 500 500 500 500 1.9 0.98 15.6 0.98 Open in a separate window a Organisms were exposed for 90 min to H 2 O 2 at the MIC prior to 18 h of anaerobic growth in MRS broth, pH 6.5. b Median of four repetitions. c Amp, ampicillin; Clo, cloxacillin; Pen, penicillin G; Van, vancomycin; Kan, kanamycin; Neo, neomycin; Par, paromomycin; Str, streptomycin; Chl, chloramphenicol; Ery, erythromycin; Tet, tetracycline hydrochloride; Nis, nisin A. MICs of antibiotics for hydrogen peroxide-stressed a bifidobacteria The MICs of vancomycin and kanamycin did not change for any strain after H 2 O 2 treatment.

Techniques:

Journal:

Article Title: Antibiotic Susceptibility Profile of Bifidobacteria as Affected by Oxgall, Acid, and Hydrogen Peroxide Stress ▿

doi: 10.1128/AAC.00261-06

Figure Lengend Snippet: MICs of antibiotics for bifidobacteria after short-term oxgall stress a

Article Snippet: Meanwhile, one strain developed slight resistance to ampicillin and two developed slight resistance to cloxacillin, but none developed resistance to penicillin G. table ft1 table-wrap mode="anchored" t5 TABLE 5. caption a7 Organism MIC (μg/ml) b of the following antibiotic c for the indicated organism: Amp Clo Pen Van Kan Neo Par Str Chl Ery Tet Nis B. animalis ATCC 27536 3.9 3.9 0.98 >500 500 500 500 250 3.9 0.98 62.5 0.98 B. bifidum R 0071 0.98 31.2 0.98 >500 500 125 125 250 1.9 500 62.5 0.98 B. bifidum ATCC 15696 Did not survive H 2 O 2 stressing B. bifidum BB12 0.98 1.9 0.98 >500 500 250 500 250 1.9 0.98 7.8 0.98 B. breve ATCC 15700 3.9 3.9 0.98 >500 >500 250 500 500 1.9 0.98 31.2 0.98 B. breve R0070 0.98 15.6 0.98 >500 500 250 250 250 1.9 0.98 7.8 0.98 B. infantis ATCC 15697 Did not survive H 2 O 2 stressing B. longum ATCC 15707 0.98 0.98 0.98 >500 500 250 500 62.5 1.9 0.98 7.8 0.98 B. longum ATCC 15708 3.9 7.8 0.98 >500 500 250 250 500 3.9 0.98 31.2 0.98 B. longum R0175 1.9 31.2 0.98 >500 500 125 250 250 1.9 0.98 15.6 1.9 B. longum P/N 601377 0.98 31.2 0.98 >500 250 125 125 125 1.9 1.9 125 0.98 B. pseudolongum ATCC 25562 Did not survive H 2 O 2 stressing B. thermophilum ATCC 25866 3.9 7.8 0.98 >500 500 500 500 500 1.9 0.98 15.6 0.98 Open in a separate window a Organisms were exposed for 90 min to H 2 O 2 at the MIC prior to 18 h of anaerobic growth in MRS broth, pH 6.5. b Median of four repetitions. c Amp, ampicillin; Clo, cloxacillin; Pen, penicillin G; Van, vancomycin; Kan, kanamycin; Neo, neomycin; Par, paromomycin; Str, streptomycin; Chl, chloramphenicol; Ery, erythromycin; Tet, tetracycline hydrochloride; Nis, nisin A. MICs of antibiotics for hydrogen peroxide-stressed a bifidobacteria The MICs of vancomycin and kanamycin did not change for any strain after H 2 O 2 treatment.

Techniques:

Journal:

Article Title: Antibiotic Susceptibility Profile of Bifidobacteria as Affected by Oxgall, Acid, and Hydrogen Peroxide Stress ▿

doi: 10.1128/AAC.00261-06

Figure Lengend Snippet: MICs of antibiotics for hydrogen peroxide-stressed a bifidobacteria

Article Snippet: Meanwhile, one strain developed slight resistance to ampicillin and two developed slight resistance to cloxacillin, but none developed resistance to penicillin G. table ft1 table-wrap mode="anchored" t5 TABLE 5. caption a7 Organism MIC (μg/ml) b of the following antibiotic c for the indicated organism: Amp Clo Pen Van Kan Neo Par Str Chl Ery Tet Nis B. animalis ATCC 27536 3.9 3.9 0.98 >500 500 500 500 250 3.9 0.98 62.5 0.98 B. bifidum R 0071 0.98 31.2 0.98 >500 500 125 125 250 1.9 500 62.5 0.98 B. bifidum ATCC 15696 Did not survive H 2 O 2 stressing B. bifidum BB12 0.98 1.9 0.98 >500 500 250 500 250 1.9 0.98 7.8 0.98 B. breve ATCC 15700 3.9 3.9 0.98 >500 >500 250 500 500 1.9 0.98 31.2 0.98 B. breve R0070 0.98 15.6 0.98 >500 500 250 250 250 1.9 0.98 7.8 0.98 B. infantis ATCC 15697 Did not survive H 2 O 2 stressing B. longum ATCC 15707 0.98 0.98 0.98 >500 500 250 500 62.5 1.9 0.98 7.8 0.98 B. longum ATCC 15708 3.9 7.8 0.98 >500 500 250 250 500 3.9 0.98 31.2 0.98 B. longum R0175 1.9 31.2 0.98 >500 500 125 250 250 1.9 0.98 15.6 1.9 B. longum P/N 601377 0.98 31.2 0.98 >500 250 125 125 125 1.9 1.9 125 0.98 B. pseudolongum ATCC 25562 Did not survive H 2 O 2 stressing B. thermophilum ATCC 25866 3.9 7.8 0.98 >500 500 500 500 500 1.9 0.98 15.6 0.98 Open in a separate window a Organisms were exposed for 90 min to H 2 O 2 at the MIC prior to 18 h of anaerobic growth in MRS broth, pH 6.5. b Median of four repetitions. c Amp, ampicillin; Clo, cloxacillin; Pen, penicillin G; Van, vancomycin; Kan, kanamycin; Neo, neomycin; Par, paromomycin; Str, streptomycin; Chl, chloramphenicol; Ery, erythromycin; Tet, tetracycline hydrochloride; Nis, nisin A. MICs of antibiotics for hydrogen peroxide-stressed a bifidobacteria The MICs of vancomycin and kanamycin did not change for any strain after H 2 O 2 treatment.

Techniques: